Generator
Part:BBa_K624041
Designed by: Yi-Hong Liou Group: iGEM11_NYMU-Taipei (2011-10-05)
pT7-tetO+RBS+minC+mcherry
This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter. pT7+RBS+tetR+RBS+GFP+Ter (BBa_K624002) regulates this construct by producing tet repressor in the presence of tetracycline.
MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 259
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |