Generator

Part:BBa_K624041

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

pT7-tetO+RBS+minC+mcherry

This construct is composed of pT7-tetO, ribosome binding site, minC cell division inhibitor, and mcherry reporter. pT7+RBS+tetR+RBS+GFP+Ter (BBa_K624002) regulates this construct by producing tet repressor in the presence of tetracycline.

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 259
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None